THE EFFECT OF THE ETHANOLIC EXTRACT OF ANCHOMANES DIFFORMIS (BLUME) ON LIVER FUNCTIONS OF ADULT WISTAR RATS

  • Type: Project
  • Department: Bio-Chemistry
  • Project ID: BCH0037
  • Access Fee: ₦5,000 ($14)
  • Chapters: 5 Chapters
  • Pages: 65 Pages
  • Format: Microsoft Word
  • Views: 1.4K
  • Report This work

For more Info, call us on
+234 8130 686 500
or
+234 8093 423 853

ABSTRACT

This research was carried out to determine the effect of administration of Achomanes Difformis (blume) on liver functions in adult wistar rat, in which 20 adult wistar rats were purchased at the institute of advanced medical research and training (IMRAT), UCH, Ibadan. The rats were grouped into 4 in which there is a control group in which the rats were placed on normal diet and water. Other groups (A, B and C) were administered 300mg/kg/b.wt, 500mg/kg/b.wt and 700mg/kg/b.wt respectively. The herb was administered for duration of 14 days. Their cages were cleaned every day maintain proper hygiene. The rats were sacrificed immediately after two weeks of drug administration. From the result obtained, the administration of Anchomanes difformis has a hepatotoxic effect. It showed that there was an insignificant increase (p>0.05) in the level of AST, ALT and ALP, and an insignificant decrease (p>0.05) in the level of Total and Direct Bilirubin in the plasma at different dosage rate of the herb, when compared to the control group. The result demonstrates that the ingestion of Anchomanes difformis has a hepatotoxic effect in adult wistar rat.

TABLE OF CONTENTS

Abstract                                                                                              

CHAPTER ONE

1.0   Introduction

1.1   Medicinal plants

1.1.2 History of medicinal plants

1.2   Liver function test

1.3   Aim of study

CHAPTER TWO

2.0  Literature review

2.1  Anchomanes Difformis                                                                  

2.1.2 Features

2.1.3 Uses of Anchomanes Difformis

2.1.4 Scientific studies on A.difformis

2.2 The Liver

2.2.1 Anatomy of the Liver

2.2.2 Functions

2.2.3 Cell types

2.3 Evaluation of Liver functions

2.4 Abnormal levels of liver enzymes

2.4.1 Nonalcoholic fatty liver damage

2.4.2 Alcohol

2.4.3 Wilson disease

2.4.4 Hepatits

2.4.5 Cirhosis

2.4.6 Medications

2.5 Aspartate aminotransferase (AST)

2.5 Functions

2.5.1 Mechanism of action

2.5.2 Clinical signifance

2.6 Alanine aminotransferase (ALT)

2.6.1 Functions

2.6.2 Clinical significance

2.7 Alkaline phosphatase (ALP)

2.7.1 Physiology

2.7.2 Elevation in ALP level

2.8 Bilirubin

2.8.1 Functions

2.8.2 Clinical significance

2.8.3 Abnormal Bilirubin level

CHAPTER THREE

3.0 Materials and methods

3.1 Apparatus and equipment

3.2 Chemical reagents

3.3 Collection and extraction of plant materials

3.4 Practical history and Animal management

3.5 Drug administration

3.6 Sacrificing of rats

3.7 Organ homogenization

3.8 Biochemical analyses

3.8.1 Bilirubin

3.8.1.1 Principle

3.8.1.2 Reagent composition

3.8.1.3 Procedure

3.8.2 Alanine aminotransferase

3.8.2.1 Principle

3.8.2.2 Reagent composition

3.8.2.3 Procedure

3.8.2.4 Calculation

3.8.3 Alkaline phosphatase

3.8.3.1 Principle

3.8.3.2 Reagent composition

3.8.3.3 Preparation of reagents

3.8.3.4 Procedure

3.8.3.5 Calculation

3.8.4 Aspartate aminotransferase

3.8.4.1 Principle

3.8.4.2 Reagent composition

3.8.4.3 Procedure

3.8.4.4 Calculation

CHAPETER FOUR

4.0 Results

CHAPTER FIVE

5.0 Discussion, conclusion and recommendation

5.1 Discussion

5.2 Conclusion

5.3 Recommendation

REFERENCE                                                                                  

APPENDIX                                                                                          

 LIST OF TABLES

TABLE 1: Mean concentration of Aspartate aminotransferase.

TABLE 2: Mean concentration of Alanine aminotransferase.

TABLE 3: Mean concentration of Alkaline phosphatase.

TABLE 4: Mean concentration of Direct bilirubin.

TABLE 5: Mean concentration of Total bilirubin.

LIST OF FIGURES

FIGURE 1: Fresh plant of Anchomanes difformis.

FIGURE 2: Rhizome of Anchomanes difformis.                                  

FIGURE 3: Labeled structure of the liver.

FIGURE 4: Bar chart showing the level of Aspartate aminotransferase in adult wistar rats.

FIGURE 5: Bar chart showing the level of Alanine aminotransferase in adult wistar rats.

FIGURE 6: Bar chart showing the level of Alkaline phosphatase in adult wistar rats.

FIGURE 7: Bar chart showing the level of Direct bilirubin in adult wistar rats.

FIGURE 8: Bar chart showing the level of Total bilirubin in adult wistar rats.

THE EFFECT OF THE ETHANOLIC EXTRACT OF ANCHOMANES DIFFORMIS (BLUME) ON LIVER FUNCTIONS OF ADULT WISTAR RATS
For more Info, call us on
+234 8130 686 500
or
+234 8093 423 853

Share This
  • Type: Project
  • Department: Bio-Chemistry
  • Project ID: BCH0037
  • Access Fee: ₦5,000 ($14)
  • Chapters: 5 Chapters
  • Pages: 65 Pages
  • Format: Microsoft Word
  • Views: 1.4K
Payment Instruction
Bank payment for Nigerians, Make a payment of ₦ 5,000 to

Bank GTBANK
gtbank
Account Name Obiaks Business Venture
Account Number 0211074565

Bitcoin: Make a payment of 0.0005 to

Bitcoin(Btc)

btc wallet
Copy to clipboard Copy text

500
Leave a comment...

    Details

    Type Project
    Department Bio-Chemistry
    Project ID BCH0037
    Fee ₦5,000 ($14)
    Chapters 5 Chapters
    No of Pages 65 Pages
    Format Microsoft Word

    Related Works

    ABSTRACT This research was carried out to determine the effect of administration of  Achomanes Difformis (blume)  on liver functions in adult wistar rat, in which 20 adult wistar rats were purchased at the institute of advanced medical research and training (IMRAT), UCH, Ibadan. The rats were grouped into 4 in which there is a control group in... Continue Reading
    Abstract Piper  guineense   ( Uziza  in Igbo) is a medicinal plant whose leaves are commonly used as spices.   This study is to investigate the effects of the  ethanolic  leaf extracts of  P. guineense   on the h istology of the liver , liver biom arker enzymes  of  paraquat -induced Thirty (30) adult male  Wistar  rats w eighing... Continue Reading
    Abstract Areca catechu (also known as betel nut) is a common masticatory in tropical and subtropical countries.  Its seeds contain condensed tannins (Procyanidins) called arecatannins, which are reported to be carcinogenic. Virgin Coconut Oil is a colourless to brown-yellow edible oil obtained from fresh, mature kernel of Cocos nucifera... Continue Reading
    The overall objective of this study is to scientifically evaluate the claimed fertility enhancing potential and to assess the metabolic effect of the ethanolic extract of   Sphenocentrum jollyanum pierre (Menispermaceae) root using male wistar rats as model. The specific objectives include the following ·          To determine the... Continue Reading
    CHAPTER ONE 1.0 INTRODUCTION Procreation is an important moral and religious issue and aphrodisiacs are sought to ensure both male and female potency. Sexual potency is part of the male ego, the anxiety and the humiliation that are associated with a declining sexual ability are common to all cultures. That is why the popularity of aphrodisiacs has... Continue Reading
    The overall objective of this study is to scientifically evaluate the claimed fertility enhancing potential and to assess the metabolic effect of the ethanolic extract of Sphenocentrum jollyanum pierre (Menispermaceae) root using male wistar rats as model. The specific objectives include the following · To determine the proximate and... Continue Reading
    CHAPTER ONE 1.0    INTRODUCTION Procreation is an important moral and religious issue and aphrodisiacs are sought to ensure both male and female potency. Sexual potency is part of the male ego, the anxiety and the humiliation that are associated with a declining sexual ability are common to all cultures. That is why the popularity of... Continue Reading
    Abstract Present investigations were carried out on the effect of ethanol extract of Desmodium velutinum stem on some liver enzymes of albino wistar rats fed with high fat from cow‟s brain. Twelve healthy albino wistar rats were divided into four groups. Group I rats were fed with balanced diet (normal feed, that is grower‟s mash) and tap... Continue Reading
    Abstract Present investigations were carried out on the effect of ethanol extract of Desmodium velutinum stem on some liver enzymes of albino wistar rats fed with high fat from cow‟s brain. Twelve healthy albino wistar rats were divided into four groups. Group I rats were fed with balanced diet (normal feed, that is grower‟s mash) and tap... Continue Reading
    Abstract Present investigations were carried out on the effect of ethanol extract of Desmodium velutinum stem on some liver enzymes of albino wistar rats fed with high fat from cow‟s brain. Twelve healthy albino wistar rats were divided into four groups. Group I rats were fed with balanced diet (normal feed, that is grower‟s mash) and tap... Continue Reading
    Call Us
    whatsappWhatsApp Us